Researchers develop random priming amplification method for whole-genome sequencing of SARS-CoV-2 and H1N1

By | July 5, 2021
Researchers from the UK recently compared the performance of 4 different random priming amplification methods to recover SARS-CoV-2 RNA. In method 1 (H-P), the reverse transcriptase (RT) step included random hexamers, whereas, in methods 2-4, RT incorporated an octamer primer with a known tag. This study is published on the bioRxiv preprint server.